SHORT COMMUNICATION The non-specific binding of tyrosine kinase inhibitors to human liver microsomes
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چکیده
240 words Introduction: 421 words Results and Discussion: 1795 words Abbreviations: CMC, critical micelle concentration; HLM, human liver microsomes; HPLC, high performance liquid chromatography; NSB, non-specific binding; RED, rapid equilibrium dialysis; TKI, tyrosine kinase inhibitor This article has not been copyedited and formatted. The final version may differ from this version. DMD Fast Forward. Published on October 6, 2015 as DOI: 10.1124/dmd.115.065292 at A PE T Jornals on Jne 3, 2017 dm d.aspurnals.org D ow nladed from
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متن کاملShort Communication The Nonspecific Binding of Tyrosine Kinase Inhibitors to Human Liver Microsomes
Drugs and other chemicals frequently bind nonspecifically to the constituents of an in vitro incubation mixture, particularly the enzyme source [e.g., human livermicrosomes (HLM)]. Correction for nonspecific binding (NSB) is essential for the accurate calculation of the kinetic parameters Km, Clint, and Ki. Many tyrosine kinase inhibitors (TKIs) are lipophilic organic bases that are nonionized ...
متن کاملShort Communication The Nonspecific Binding of Tyrosine Kinase Inhibitors to Human Liver Microsomes
Drugs and other chemicals frequently bind nonspecifically to the constituents of an in vitro incubation mixture, particularly the enzyme source [e.g., human livermicrosomes (HLM)]. Correction for nonspecific binding (NSB) is essential for the accurate calculation of the kinetic parameters Km, Clint, and Ki. Many tyrosine kinase inhibitors (TKIs) are lipophilic organic bases that are nonionized ...
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